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商品編號


EXOCET96A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome quantitation


商品信息

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Overview:
Move quickly with
SBI
’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes
1, 2
(using mass spectrometry and Western blotting,
SBI
has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).


EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.


Fast—complete in 20 minutes

Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes

Quantitative—included calibration standards enable calculation of the number of exosome particles

Flex
IBL
e—works with all mammalian species tested (human, mouse, rat)

Fully compat
IBL
e—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography


Choose the exosome quantitation method that’s best for your studies




?

Exo
ELISA
-ULTRA CD63 Detection

Exo
ELISA
CD9
Exo
ELISA
CD63
Exo
ELISA
CD81

EXOCET

FluoroCet





Use

For fast and sensitive antibody-based quantitation of exosomes

For sensitive quantitation of exosomes when time and input sample are not limiting

For fast quantitation of extracellular vesicles with moderate sample input requirements

For the most sensitive quantitation of extracellular vesicles with very low sample input requirements



Detection method

Antibody

Antibody

Enzymatic

Enzymatic



Quantitation chemistry

Enzymatic (HRP)

Enzymatic (HRP)

Colorimetric

Fluorescent



Total protocol time

4 hours (no overnight incubation)

24 hours

20 min

60 min



Input sample amount (protein equivalent)

1 – 200 ?g

>500 ?g

50 ?g

<1 ?g





REFERENCES


Savina A,
et al
. The exosome pathway in K562 cells is regulated by Rab11.
J Cell Sci
. 2002 Jun 15.
115
(Pt 12):2505-15. PMID: 12045221.

Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway.
Am J Physiol Heart Circ Physiol
. 2007 Jun.
292
(6):H3052-6. PMID: 17307989.


How It Works:
Exosome quantitation with EXOCET is quick and easy

Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods) Lyse exosomes with the included Exosome Lysis Buffer Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm
Citations:
Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis.
Mediators Inflamm.
. 2017 Jun 21; 2017:4814987. PM ID: 28634420
Wu, X, et al. (2017) Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-ββ1-Containing Exosomes from GECs to GMCs Read More: http://www.worldscientific.com/doi/abs/10.1142/S0192415X17500586.
The American Journal of Chinese Medicine
. 2017 Jun 29;. Link: The American Journal of Chinese Medicine
Rody, WJ, et al. (2017) The use of cell culture platforms to identify novel
Marker
s of bone and dentin resorption.
Orthod Craniofac Res
. 2017 Jun 1;:89-94. PM ID: 28643914
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology.
Pediatr. Res.
. 2017 Apr 5;. PM ID: 28157838
Slowey, PD. (2017) Salivary Diagnostics Using Purified Nucleic Acids.
Methods Mol. Biol.
. 2017 Dec 7; 1537:3-15. PM ID: 27924585
Sharma, R, et al. (2017) Detection of phosphatidylserine-positive exosomes for the diagnosis of early-stage malignancies.
Br. J. Cancer
. 2017 Jun 22;. PM ID: 28641308
Hurwitz, SN, et al. (2017) CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-κB Signaling.
J. Virol.
. 2017 Mar 1; 91(5). PM ID: 27974566
Thomou, T, et al. (2017)
ADI
pose-derived circulating miRNAs regulate gene expression in other tissues.
Nature
. 2017 Feb 23; 542(7642):450-455. PM ID: 28199304
Jiang, X, et al. (2017) Exosomes from pediatric dilated cardiomyopathy patients modulate a pathological response in cardiomyocytes.
Am. J. Physiol. Heart Circ. Physiol.
. 2017 Apr 1; 312(4):H818-H826. PM ID: 28130338
Hovsepian, T, Saint-Auret, G & Lebot, C. (2017) A Skin Extracellular Signaling Pathway via Intercellular Exosomes Highlighted by Genetic and Epigenetic Analysis Applied to a Natural Ingredient.
29th IFSCC Congress
. ;:23-28. Link: 29th IFSCC Congress
Colluru, VT. (2016) Anti-Tumor DNA vaccination: Optimizing Antigen Expression and Presentation.
Thesis
. ;. Link: Thesis
Yang, J, et al. (2016) Anomalous uptake and circulatory characteristics of the plant-based small RNA MIR2911.
Sci Rep
. 2016 Jun 2; 6:26834. PM ID: 27251858
Lee, JY, et al. (2016) Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response.
Arthritis Res. Ther.
. 2016 Nov 16; 18(1):264. PM ID: 27852323
Huynh, N, et al. (2016) Characterization of Regulatory Extracellular Vesicles from Osteoclasts.
J. Dent. Res.
. 2016 Jun 1; 95(6):673-9. PM ID: 26908631
Gutierrez, MJ, et al. (2016) Airway Secretory microRNAome Changes during Rhinovirus Infection in Early Childhood.
PLoS ONE
. 2016 Sep 20; 11(9):e0162244. PM ID: 27643599
Rossaint, J, et al. (2016) Directed transport of neutrophil-derived extracellular vesicles enables platelet-mediated innate immune response.
Nat Commun
. 2016 Nov 15; 7:13464. PM ID: 27845343
Matsumoto, Y, et al. (2016) Quantification of plasma exosome is a potential prognostic
Marker
for esophageal squamous cell carcinoma.
Oncol. Rep.
. 2016 Nov 1; 36(5):2535-2543. PM ID: 27599779
Wu, XM, et al. (2016) Exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells to promote renal fibrosis.
Biol Open
. 2016 Apr 15; 5(4):484-91. PM ID: 27010029
Basappa, SN. (2015) Effect of cmvIL-10 on Exosome Production by Human Breast Cancer Cells.
Thesis
. ;. Link: Thesis
Peterson, MF, et al. (2015) Integrated systems for exosome investigation.
Methods
. 2015 Oct 1; 87:31-45. PM ID: 25916618