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商品編號


FCET96A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome quantitation


商品信息

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Overview:
Get ultrasensitive exosome quantitation when samples are limiting

Enabling highly sensitive quantitation of exosomes in only 60 minutes, the FluoroCet
?
Kit takes our popular EXOCET assay and increases the sensitivity over 30-fold. Fully compat
IBL
e with all of
SBI
’s ExoQuick
?
exosome isolation reagents as well as ultracentrifugation, FluoroCet is great for quantifying exosomes when samples are limiting.



Sensitive

Requires as little as 500 ng of exosomal protein

Perfect for clinical samples or any situation where samples are limiting



Fast—assay is complete in only 60 minutes

Versatile—compat
IBL
e with all major exosome isolation methods (
e.g.
ExoQuick, ultracentrifugation, ultrafiltration, and immunoffinity capture) from human, mouse, and rat samples

Quantitative—internal assay standards enable calculation of exosome abundance


Like EXOCET, FluoroCet measures the activity of acetecylcholinesterase (AChE), a known exosome protein, although the more sensitive FluoroCet assay relies on readout of fluorescence emission at 590-600 nm. To enable calculation of exosome abundance in your target sample, each kit includes standards calibrated to known amounts of human exosomes as determined by NanoSight analysis. The FluoroCet Kit contains all of the reagents needed to perform 96 reactions.

Choose the exosome quantitation method that’s best for your studies




?

Exo
ELISA
-ULTRA CD63 Detection

Exo
ELISA
CD9
Exo
ELISA
CD63
Exo
ELISA
CD81

EXOCET

FluoroCet





Use

For fast and sensitive antibody-based quantitation of exosomes

For sensitive quantitation of exosomes when time and input sample are not limiting

For fast quantitation of extracellular vesicles with moderate sample input requirements

For the most sensitive quantitation of extracellular vesicles with very low sample input requirements



Detection method

Antibody

Antibody

Enzymatic

Enzymatic



Quantitation chemistry

Enzymatic (HRP)

Enzymatic (HRP)

Colorimetric

Fluorescent



Total protocol time

4 hours (no overnight incubation)

24 hours

20 min

60 min



Input sample amount (protein equivalent)

1 – 200 ?g

>500 ?g

50 ?g

<1 ?g





How It Works:
Exosome quantitation with FluoroCet is quick and easy

Calibrate your fluorescence plate reader Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods) Lyse exosomes with the included Exosome Lysis Buffer Measure AChE activity—add the included buffer and incubate for 20 minutes (or appropriate time as determined during the calibration step) at room temperature in the dark, and then measure fluorescence using excitation at 530–570 nm and emission at 590–600 nm.