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商品編號


CYTO120-PA-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome labeling





商品信息

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Overview:
Easily track exosomes with a CD63-GFP fusion

Monitor exosome dynamics with the pCT-CD63-GFP (pCMV) Exosome Cyto-Tracer (also available as a CD63-RFP fusion, pCT-CD63-RFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses GFP to the tetraspanin CD63, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce GFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.



Stable lentivector-based system

Ideal for co-localization studies

Monitor exosome dynamics and functional studies in real time

Label exosomes from primary cells, tumor cells, stem cells, and more


Choose the exosome quantitation method that’s best for your studies




Promoter (Cat.#)

Cyto-Tracer

Target Location

Peptide Tag





CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)

pCT-Mem-GFP

Plasma Membrane

Neuromodulin



CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)

pCT-InnerMem-GFP

Inner Leaflet of Membrane

Farnesylation signal



CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)

pCT-Mito-GFP

Mitochondria

Cytochrome C oxidase VIII



CMV (CYTO103-PA-1/CYTO103-VA-1)

pCT-ER-GFP

Endoplasmic Reticulum

ER targeting consensus



CMV (CYTO104-PA-1/CYTO104-VA-1)

pCT-Golgi-GFP

Golgi Apparatus

Galactosyltrasferase



CMV (CYTO105-PA-1/CYTO105-VA-1)

pCT-Secretory-GFP

ER-Golgi? Vesicles

Secretory consensus peptide



CMV (CYTO106-PA-1/CYTO106-VA-1)

pCT-Lyso-GFP

Lysosome

Cathepsin B



CMV (CYTO107-PA-1/CYTO107-VA-1)

pCT-Endo-GFP

Endosome

RhoB



CMV (CYTO107R-PA-1/CYTO107R-VA-1)

pCT-Endo-RFP

Endosome

RhoB



CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)

pCT-Pero-GFP

Peroxisome

Peroxisomal consensus signal



CMV (CYTO120-PA-1/CYTO120-VA-1)

pCT-Autophago-GFP

Autophagosome

LC3



CMV (CYTO120R-PA-1/CYTO120R-VA-1)

pCT-Autophago-RFP

Autophagosome

LC3



CMV (CYTO109-PA-1/CYTO109-VA-1)

pCT-CD63-GFP

Exosome/Secretory

CD63 Tetraspanin



CMV (CYTO109R-PA-1/CYTO109R-VA-1)

pCT-CD63-RFP

Exosome/Secretory

CD63 Tetraspanin



CMV (CYTO122-PA-1/CYTO122-VA-1)

pCT-CD9-GFP

Exosome/Secretory

CD9 Tetraspanin



CMV (CYTO123-PA-1/CYTO123-VA-1)

pCT-CD9-RFP

Exosome/Secretory

CD9 Tetraspanin



CMV (CYTO124-PA-1/CYTO124-VA-1)

pCT-CD81-GFP

Exosome/Secretory

CD81 Tetraspanin



CMV (CYTO125-PA-1/CYTO125-VA-1)

pCT-CD81-RFP

Exosome/Secretory

CD81 Tetraspanin



CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)

pCT-Actin-GFP

Cytoskeleton

β-actin



CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)

pCT-Tublin-GFP

Cytoskeleton

α-tublin



CMV (CYTO111R-PA-1/CYTO111R-VA-1)

pCT-Tublin-RFP

Cytoskeleton

α-tublin



CMV (CYTO112-PA-1/CYTO112-VA-1)

pCT-MAP4-GFP

Microtubule

Microtubule associated protein 4 (MAP4)



CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)

pCT-H2B-GFP

Nucleus

H2B



CMV (CYTO114-PA-1/CYTO114-VA-1)

pCT-Apoptosis-Luc

?Caspase 3/7 activation

Cyclic inactive luciferase



CMV (CYTO115-PA-1/CYTO115-VA-1)

pCT-GFP-BAX

Cytosol to Mitochondria

BAX



CMV (CYTO116-PA-1/CYTO116-VA-1)

pCT- Catenin-GFP

Cytosol to Nucleus/Cell Membrane

β-Catenin activation



CMV (CYTO117-PA-1/CYTO117-VA-1)

pCT-CMV-PSD95-GFP

Dendrite Membranes

PSD-95



CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)

pCT-Cyto-GFP

Cytosolic Compartment

GFP-Untagged



CMV (CYTO119-PA-1/CYTO119-VA-1)

pCT-Cyto-RFP

Cytosolic Compartment

RFP-Untagged



MSCV (CYTO121-PB-1/CYTO121-VB-1)

pCT-Cyto-GFpLuc

GFP and Luciferase Control

GFP and Luciferase (Untagged)




Supporting Data:

See some of our exosome Cyto-Tracers in action

The following figure and videos are from:

Garcia NA,
et al
. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells.
PLoS ONE
. 2015.
10
(9). PMCID: PMC4578916.




Figure 4 from Garcia,
et al
. Exosome transfer from CMs to ECs.
(A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.





Video S1 from Garcia,
et al
. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.





Video S2 from Garcia,
et al
. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.




Labeled exosomes.
(Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-Tracers co-transfected into HEK293 cells.


Citations:
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase.
J. Biol. Chem.
. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
Gonzalez-King, H, et al. (2017) Hypoxia Induc
IBL
e Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes.
Stem Cells
. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival.
Carcinogenesis
. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular.
Thesis
. ;. Link: Thesis
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes.
Tumour Biol.
. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie.
Thesis
. ;. Link: Thesis
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation.
Nat Commun
. 2015 Jun 24; 6:7506. PM ID: 26103923
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI.
Thesis
. ;. Link: Thesis
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway.
Thesis
. ;. Link: Thesis
Sato, Y, et al. (2014) MTCL1 crosslinks and st
ABI
lizes non-centrosomal microtubules on the Golgi membrane.
Nat Commun
. 2014 Nov 4; 5:5266. PM ID: 25366663
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment.
J. Biol. Chem.
. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β.
Nat Commun
. 2014 Nov 26; 5:5513. PM ID: 25423885