SBI/XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector/XPAK510PA-1/10 ?g
基因編輯
商品編號
XPAK510PA-1
品牌
SBI
公司
System Biosciences(SBI)
公司分類(lèi)
exosome labeling
商品信息
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Overview:
Turn your protein into exosome cargo with XPack
TM
Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.
XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector
With the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use
SBI
’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong CMV promoter delivers high expression levels in most cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with an MSCV promoter for expression in hematopoietic and stem cells).
The XPack System at a glance:
Powerful—enables a wealth of applications, including
Exosome labeling
Exosome cargo tracking
Exosomes as cargo delivery vehicles
Exosomes as biotherapeutics
Exosome-generating stable cell lines with protein cargo-of-interest
Optimized—
SBI
developed the XPack peptide tag for robust exosome targeting
Reliable—based on
SBI
’s highly-cited, well-proven lentivector technology
Versatile
Two promoter options for expression in a wide range of cells
Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
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CMV Promoter
For most cell lines
MSCV Promoter
For hematopoietic and stem cell lines
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Customize with our cloning vector
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XPAK510PA-1
XPack CMV-XP-MCS-EF1α-Puro
XPAK710PA-1
XPack MSCV-XP-MCS-EF1α-Puro
Label exosomes with pre-built reporters
GFP
XPAK530CL-1
XPAK530PA-1
XPAK530VA-1
XPack CMV-XP-GFP-EF1α-Puro
XPAK730PA-1
XPAK730VA-1
XPack MSCV-XP-GFP-EF1α-Puro
RFP
XPAK531PA-1
XPack CMV-XP-RFP-EF1α-Puro
XPAK731PA-1
XPack MSCV-XP-RFP-EF1α-Puro
Luciferase
XPAK532CL-1
XPAK532PA-1
XPAK532VA-1
XPack CMV-XP-Luciferase-EF1α-Puro
XPAK732PA-1
XPAK732VA-1
XPack MSCV-XP-Luciferase-EF1α-Puro
Supporting Data:
Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology
Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).
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Figure 1. XPack-CDKN1B exosomes reduce cellular proliferation.
METHODS
: We cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC?. We then added 5, 50, or 100 ?g of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (
Millipore
catalog # CT02) and normalilzed to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation.
產(chǎn)品貨號:7112.8