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商品編號


XPAK532PA-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome labeling



商品信息

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Overview:
Label exosomes with luciferase using XPack
TM

Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of
SBI
’s well-proven lentivector technology to express luciferase from the strong CMV promoter (also available with an MSCV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.


The XPack System at a glance:


Powerful—enables a wealth of applications, including

Exosome labeling

Exosome cargo tracking

Exosomes as cargo delivery vehicles

Exosomes as biotherapeutics

Exosome-generating stable cell lines with protein cargo-of-interest



Optimized—
SBI
developed the XPack peptide tag for robust exosome targeting

Reliable—based on
SBI
’s highly-cited, well-proven lentivector technology

Versatile

Two promoter options for expression in a wide range of cells

Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors




Choose the XPack Lentivector that’s right for you




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CMV Promoter
For most cell lines

MSCV Promoter
For hematopoietic and stem cell lines

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Customize with our cloning vector



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XPAK510PA-1

XPack CMV-XP-MCS-EF1α-Puro

XPAK710PA-1

XPack MSCV-XP-MCS-EF1α-Puro



Label exosomes with pre-built reporters



GFP

XPAK530CL-1
XPAK530PA-1

XPAK530VA-1

XPack CMV-XP-GFP-EF1α-Puro

XPAK730PA-1
XPAK730VA-1

XPack MSCV-XP-GFP-EF1α-Puro



RFP

XPAK531PA-1

XPack CMV-XP-RFP-EF1α-Puro

XPAK731PA-1

XPack MSCV-XP-RFP-EF1α-Puro



Luciferase

XPAK532CL-1
XPAK532PA-1

XPAK532VA-1

XPack CMV-XP-Luciferase-EF1α-Puro

XPAK732PA-1
XPAK732VA-1

XPack MSCV-XP-Luciferase-EF1α-Puro




Supporting Data:

XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells


Part 1: Packaging luciferase into exosomes with XPack

The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC
?
and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.


Figure 1. XPack efficiently packages luciferase into exosomes.

Part 2: XPack-Luciferase Exosome Delivery to Target Cells

The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.

Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.