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商品編號


XPAK710PA-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome labeling



商品信息

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Overview:
Turn your protein into exosome cargo with XPack
TM

Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.



XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector

With the XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use
SBI
’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong MSCV promoter delivers high expression levels in hematopoietic and stem cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with a CMV promoter for expression in hematopoietic and stem cells).

The XPack System at a glance:


Powerful—enables a wealth of applications, including

Exosome labeling

Exosome cargo tracking

Exosomes as cargo delivery vehicles

Exosomes as biotherapeutics

Exosome-generating stable cell lines with protein cargo-of-interest



Optimized—
SBI
developed the XPack peptide tag for robust exosome targeting

Reliable—based on
SBI
’s highly-cited, well-proven lentivector technology

Versatile

Two promoter options for expression in a wide range of cells

Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors




Choose the XPack Lentivector that’s right for you




?

CMV Promoter
For most cell lines

MSCV Promoter
For hematopoietic and stem cell lines

?

?





Customize with our cloning vector



?

XPAK510PA-1

XPack CMV-XP-MCS-EF1α-Puro

XPAK710PA-1

XPack MSCV-XP-MCS-EF1α-Puro



Label exosomes with pre-built reporters



GFP

XPAK530CL-1
XPAK530PA-1

XPAK530VA-1

XPack CMV-XP-GFP-EF1α-Puro

XPAK730PA-1
XPAK730VA-1

XPack MSCV-XP-GFP-EF1α-Puro



RFP

XPAK531PA-1

XPack CMV-XP-RFP-EF1α-Puro

XPAK731PA-1

XPack MSCV-XP-RFP-EF1α-Puro



Luciferase

XPAK532CL-1
XPAK532PA-1

XPAK532VA-1

XPack CMV-XP-Luciferase-EF1α-Puro

XPAK732PA-1
XPAK732VA-1

XPack MSCV-XP-Luciferase-EF1α-Puro




Supporting Data:

Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology

Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).



Figure 1. XPack-CDKN1B exosomes reduce cellular proliferation.

METHODS
: We cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC?. We then added 5, 50, or 100 ?g of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (
Millipore
catalog # CT02) and normalilzed to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation.