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商品編號


EXOGP400A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome labeling




商品信息

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Overview:
See EVsbetter with reagents specifically optimized for labeling

SBI
’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow
?
-Protein Blue improves your
ABI
lity to track and localize EVs by specifically and covalently labeling internal EV proteins with a proprietary blue fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).



Specific—carefully developed to generate a robust signal specific for internal EV proteins, le
ADI
ng to very low levels of background

Compat
IBL
e—delivers robust performance on EVs isolated using all methods tested—including ExoQuick
?
, ultracentrifugation, and column-based workflows

Easy-to-use—labeling protocol is quick and straightforward

Powerful—can be used with as little as 200 ?g of EVs


ExoGlow-Protein Blue properties:
Excitation: 403 nm
Emission: 454 nm
Laser line: 405 nm
Available next generation ExoGlow EV imaging reagents




EV Component Labeled

Cat. #

Product

xcitation (nm)

Emission (nm)

Laser Line (nm)





Internal protein

EXOGP100A-1

ExoGlow
?
-Protein EV Labeling Kit (Red)

573

588

561



EXOGP300A-1

ExoGlow
?
-Protein EV Labeling Kit (Green)

511

525

488



EXOGP400A-1

ExoGlow
?
-Protein EV Labeling Kit (Blue)

403

454

405



RNA

EXOGR800A-1

ExoGlow
?
-RNA EV Labeling Kit

485

537

488



Membrane

EXOGM600A-1

ExoGlow
?
-Membrane EV Labeling Kit

465

635

488




Supporting Data:

Imaging EVs with a new level of clarity



Figure 1. ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalization of labeled EVs by the target cells.


Figure 2. Punctate staining pattern is EV-specific. The punctate staining pattern seen in Figure 1 and Panel A is due to ExoGlow-Protein-labeled EVs. When ExoGlow-Protein is added directly to target HEK203T cells (Panel B), only diffuse, non-specific staining is seen.


Figure 3. ExoGlow-Protein signal is stable for up to at least 7 days after admi
NIST
ration of labeled EVs to cells.