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商品編號


EXOGR800A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome labeling



商品信息

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Overview:
See EVs better with reagents specifically optimized for labeling

SBI
’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow
TM
-RNA improves your
ABI
lity to track and localize EVs by specifically labeling EV mRNAs with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate EV studies (see below for our full range of next generation ExoGlow reagents).



Specific—carefully developed to generate a robust signal specific for EV mRNAs, le
ADI
ng to very low levels of background

Compat
IBL
e—delivers robust performance on EVs isolated using all methods tested—including ExoQuick
?
, ultracentrifugation, and column-based workflows

Easy-to-use—labeling protocol is quick and straightforward

Powerful—can be used with as little as 50 ?g of EVs


ExoGlow-RNA properties:
Excitation: 485 nm
Emission: 537 nm
Laser line: 488 nm

Available next generation ExoGlow EV imaging reagents




EV Component Labeled

Cat. #

Product

xcitation (nm)

Emission (nm)

Laser Line (nm)





Internal protein

EXOGP100A-1

ExoGlow
?
-Protein EV Labeling Kit (Red)

573

588

561



EXOGP300A-1

ExoGlow
?
-Protein EV Labeling Kit (Green)

511

525

488



EXOGP400A-1

ExoGlow
?
-Protein EV Labeling Kit (Blue)

403

454

405



RNA

EXOGR800A-1

ExoGlow
?
-RNA EV Labeling Kit

485

537

488



Membrane

EXOGM600A-1

ExoGlow
?
-Membrane EV Labeling Kit

465

635

488




Supporting Data:

Imaging EVs with a new level of clarity



Figure 1. ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells.
We labeled HEK293T EVs with ExoGlow-RNA and followed uptake by HEK293T cells. The fluorescence signal localizes to perinuclear and cytoplasmic regions, as would be expected for mRNA.


Figure 2. ExoGlow-RNA specifically labels mRNA
. We used ExoGlow-RNA to label total RNA isolated from HEK293T cells, and then specifically depleted mRNA using polyT magnetic beads. The almost complete loss of signal in the mRNA-depleted sample demonstrates the specificity of ExoGlow-RNA dye for mRNA.