SBI/ExoGlowTM-Membrane EV Labeling Kit/EXOGM600A-1/20 Reactions
基因編輯
商品編號
EXOGM600A-1
品牌
SBI
公司
System Biosciences(SBI)
公司分類(lèi)
exosome labeling
商品信息
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Overview:
See EVs better with reagents specifically optimized for labeling
SBI
’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow
TM
-Membrane improves your
ABI
lity to track and localize EVs by specifically labeling EV membranes with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).
Specific—carefully developed to generate a robust signal specific for EV membranes, le
ADI
ng to very low levels of background
Compat
IBL
e—delivers robust performance on EVs isolated using all methods tested—including ExoQuick
?
, ultracentrifugation, and column-based workflows
Easy-to-use—labeling protocol is quick and straightforward
Powerful—can be used with as little as 1 ?g of EVs
ExoGlow-RNA properties:
Excitation: 465 nm
Emission: 635 nm
Laser line: 488 nm
Available next generation ExoGlow EV imaging reagents
EV Component Labeled
Cat. #
Product
xcitation (nm)
Emission (nm)
Laser Line (nm)
Internal protein
EXOGP100A-1
ExoGlow
?
-Protein EV Labeling Kit (Red)
573
588
561
EXOGP300A-1
ExoGlow
?
-Protein EV Labeling Kit (Green)
511
525
488
EXOGP400A-1
ExoGlow
?
-Protein EV Labeling Kit (Blue)
403
454
405
RNA
EXOGR800A-1
ExoGlow
?
-RNA EV Labeling Kit
485
537
488
Membrane
EXOGM600A-1
ExoGlow
?
-Membrane EV Labeling Kit
465
635
488
Supporting Data:
Imaging EVs with a new level of clarity
Figure 1. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells.
We labeled HEK293T EVs with ExoGlow-Membrane and followed uptake by HEK293T cells. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.
Figure 2. ExoGlow-Membrane is specific for intact membranes.
We incubated either PBS,
liposomes
,
liposomes
pre-solubilized with detergent (0.05% Tx100), EVs, or lipoproteins. Only the samples with intact membranes—
liposomes
and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes.
產(chǎn)品貨號:3204.0