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商品編號


RA800TC-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


biomarker profiling





商品信息

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Overview:
Profile exosomal RNA bio
Marker
s and more

Looking for bio
Marker
s?
SBI
’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).



Reliable, reproduc
IBL
e exoRNA isolation from most biofluids

Sensitive detection via exoRNA amplification

Excellent preparation method for qPCR

Can be used to amplify sense-strand exoRNAs for microarray construction



With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related bio
Marker
s. To simplify and standardize the isolation of RNA from exosomes,
SBI
has developed the SeraMir family of products.

The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during
CDN
A synthesis so you can go straight to qPCR.

In addition, primers for PCR amplification are included to make double-stranded
CDN
A via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.

Choose the SeraMir Kit that’s right for you




Cat. #

Name

Kit includes









ExoQuick or ExoQuick-TC

SeraMir RNA columns and reagents

SeraMir
CDN
A synthesis and amplification reagents

384-well plate miRNAs for human, mouse, or rat



RA800A-1

Complete SeraMir Exosome RNA Amplification Kit




?



RA800TC-1

Complete SeraMir Exosome RNA Amplification Kit for Media and Urine




?



RA806A-1

SeraMir Exosome RNA Purification Kit



?

?



RA806TC-1

SeraMir Exosome RNA Purification Kit for Media and Urine



?

?



RA808A-1

SeraMir Exosome RNA Purification Column Kit

?


?

?



RA820A-1

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit







RA820TC-1

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine







RA821A-1

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit







RA821TC-1

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine







RA822A-1

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit







RA822TC-1

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine








How It Works:
Go from sample to amplified exoRNAs in a single day
Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent Purify exoRNAs with SeraMir columns Tail and tag all exoRNAs for qPCR Perform second strand synthesis to generate
CDN
As for amplification and T7 IVT
The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.


Isolate serum exosomes and purify exoRNAs


Tail exoRNAs and synthesize double-tagged
CDN
A

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and
CDN
A synthesis.

Citations:
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Bio
Marker
? And Its Role for Interleukin-6 Production in Hepatocytes.
OMICS
. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics.
Orthod Craniofac Res
. 2017 Jun 1;:95-99. PM ID: 28643924
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron vi
ABI
lity and axon growth and branching.
J
Biomat
er Appl
. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation.
ESC Heart Fail
. 2017 May 1; 4(2):112-121. PM ID: 28451447
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer.
Biochem. Biophys. Res. Commun.
. 2017 Jun 13;. PM ID: 28623135
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype.
Tissue Eng Part A
. 2017 Jun 4;. PM ID: 28580875
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals.
Conference
. ;. Link: Conference
Protocol, IIEI. (2017) List of Components.
Product
. ;. Link: Product
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation.
EBioMedicine
. 2017 Feb 1; 15:163-172. PM ID: 27974246
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles.
bio-protocol
. ; 7(4). Link: bio-protocol
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology.
Pediatr. Res.
. 2017 Apr 5;. PM ID: 28157838
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence.
Abstract
. ;. Link: Abstract
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Bio
Marker
s for Multiple Sclerosis.
Ann. Neurol.
. 2017 Apr 15;. PM ID: 28411393
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response.
Oncotarget
. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
Hubal, MJ, et al. (2017) Circulating
ADI
pocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass.
Obesity (Silver Spring)
. 2017 Jan 1; 25(1):102-110. PM ID: 27883272
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine.
J. Immunol. Methods
. 2016 Feb 1; 429:39-49. PM ID: 26723490
Yan, IK, et al. (2016) Circulating Extracellular RNA
Marker
s of Liver Regeneration.
PLoS ONE
. 2016 Jul 15; 11(7):e0155888. PM ID: 27415797
Huleihel, L, et al. (2016) Matrix-bound nanovesicles within ECM bioscaffolds.
Sci Adv
. 2016 Jun 1; 2(6):e1600502. PM ID: 27386584
Kudo, M. (2016) Speaker’s Lecture Abstracts.
Liver Cancer
. ; 5(1):1–94. Link: Liver Cancer
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University..
J Extracell Vesicles
. 2016 Mar 1; 5:30924. PM ID: 26928673