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商品編號


BSPM-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


CRISPR/Cas9 On-target Detection



Description

1,000 reactions (50 μl vol)


Size

1 Ea

商品信息

2x BlueStar
TM
PCR Master Mix contains all the necessary reagents and a gel lo
ADI
ng dye. The PCR mix can be directly loaded onto a DNA gel.

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Description:

The 2X BlueStar? PCR Master Mix is a pre-mixed 2X concentrated solution containing HoTaq? DNA Polymerase, reaction buffer, gel lo
ADI
ng dye, MgCl2 and dNTPs. The HoTaq? DNA Polymerase offers hot start PCR for room temperature reaction set-up. The 2X BlueStar? PCR Master Mix is ideal for applications requiring high yields and routine PCR laboratory use, such as SNP analysis and high throughput end-point PCR.


Comparison:


Features:

High Target Yield: 2X BlueStar? PCR Master Mix is designed with a buffer system optimized for maximum robustness and exceptional polymerase performance.

Greater Specificity: Hot-start PCR prevents non-specific amplification while increasing target yield.

Enhanced Sensitivity:?The HoTaq? DNA polymerase provides superior amplification regardless of template concentration.

Convenient Format: The convenient master mix only requires the addition of PCR primers and DNA template for amplication.


Recommended Storage Condition:
Room temperature
PCR conditions:
1. Prepare the reaction at room temperature.
2. Add template, primers and H
2
O to make up ? of the desired total volume, and then add equal volume of 2x master mix.
3. The master mix contains a high fidelity DNA polymerase.
4. The elongation speed of this master mix is about 2 Kb per minute.
5. The blue dye runs at about 1 Kb position on an agarose gel.
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