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商品編號


E1700S-100 reactions



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




100 reactions



商品信息

Description:
The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of
Bst
2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both
Bst
2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compat
IBL
e with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.
NEB
’s WarmStart LAMP Kit (DNA & RNA) is compat
IBL
e with multiple detection methods
The
NEB
WarmStart LAMP Kit (DNA & RNA) includes separate fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or endpoint visualization.
NEB
’s WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and
Optigene
Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time
Thermo
cycler (
Bio-Rad
CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence.
NEB
’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the
Optigene
Master Mix.
NEB
’s WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results
Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a
Bio-Rad
CFX98 instrument at 65°C for 75 minutes.
Lucigen
? OmniAmp? RNA & DNA Kit and
Optigene
Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.
NEB
WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets
PRIMER SET
DNA TEMPLATE
HeLa?
DNA
Lyme?
DNA
B. malayi
?
DNA
E. coli

DNA
C. elegans
?
DNA
BRCAb (human)
+
?
?
?
?
CFTR (human)
+
?
?
?
?
LymO (Lyme)
?
+
?
?
?
BmHhal (
B. malayi
)
?
?
+
?
?
dnaE (
E. coli)

?
?
?
+
?
Lec10b (
C. elegans
)
?
?
?
?
+
Lec6 (
C. elegans
)
?
?
?
?
+
In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a
Thermo
cycler (
Bio-Rad
CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.
Kit Components
The following reagents are supplied with this product:
Store at (°C)
Concentration
WarmStart LAMP 2X Master Mix
-20
LAMP Fluorescent Dye (50X)
-20