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商品編號


M0538S-1,600 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




1,600 units


Concentration

8,000 units/ml


商品信息

Description:

Bst
2.0 WarmStart DNA Polymerase is an
in silico
designed homologue of
Bacillus stearo
Thermo
philus
DNA Polymerase I, Large Fragment (
Bst
DNA Polymerase, Large Fragment) with a revers
IBL
y-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the
Bst
2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase.
Bst
2.0 WarmStart DNA Polymerase contains 5?→3? DNA polymerase activity and strong strand-displacement activity but lacks 5?→3? exonuclease activity.
Bst
2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and
Thermo
st
ABI
lity compared to wild-type
Bst
DNA Polymerase, Large Fragment.
Benefits of Bst 2.0 WarmStart
Identical LAMP reactions were run either immediately after setup (solid line) or after a 2 hour incubation at 25 °C. Without the protection from Bst 2.0 WarmStart, this room temperature incubation results in variable LAMP performance. Bst 2.0 WarmStart provides more consistent amplification reaction and enables room-temperature and high-throughput setup.
Product Source
Bst
2.0 WarmStart DNA Polymerase is prepared from an
E. coli
strain that expresses the
Bst
2.0 DNA Polymerase protein from an induc
IBL
e promoter.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Isothermal Amplification Buffer Pack
-20
10X
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Notes:
Bst 2.0 WarmStart DNA Polymerase does not exhibit 3?→ 5? exonuclease activity.Reaction temperatures above 70°C are not recommended.Bst 2.0 WarmStart DNA Polymerase cannot be used for thermal cycle sequencing or PCR.Specific reaction conditions will vary for differentisothermal amplification applications. For best results, use 1X Isothermal Amplification Buffer.