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商品編號


H0110S-50 reactions



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




50 reactions


商品信息

Description:
Thermo
philic Helicase-Dependent Amplification (tHDA) is a novel method for isothermal amplification of nucleic acids. Like PCR, the tHDA reaction selectively amplifies a target sequence defined by two primers. However, unlike PCR, tHDA uses an enzyme called a helicase to separate DNA, rather than heat. This allows DNA amplification without the need for
Thermo
cycling. The tHDA reaction can also be coupled with reverse transcription for RNA analysis.
IsoAmp? II Universal tHDA kit can be used to amplify and detect short DNA sequences (70 bp - 120 bp) at a constant temperature. The kit can be used with a variety of templates, including microbial genomic DNA, viral DNA, plasmid DNA, and
CDN
A. A single copy of target DNA can be amplified by tHDA and detected by agarose gel electrophoresis when optimized primers and buffer are used.
?IsoAmp? II Universal tHDA kit is based on a second-generation
Thermo
philic Helicase-Dependent Amplification platform. The reactions supported by IsoAmp? II Universal tHDA kit include tHDA, reverse transcription HDA (RT-HDA), real-time quantitative HDA (qHDA) and real-time quantitative RT-HDA (qRT-HDA), from a single reaction buffer. IsoAmp? II Universal tHDA kit contains an enzyme mix, dNTP solution and buffer, allowing flexibility in reaction setup. A Control Template and a set of specific primers are supplied for the positive control reaction.
Recommended storage conditions for the tHDA kit are -20°C for storage shorter than 6 months and -80°C for storage greater than 6 months. Please avoid repeated freeze-thaw cycles as this may decrease performance of the kit.
Figure 1.
HDA Technology.
Figure 2.
Positive control assays with varying amount of Control Template. A, two-step tHDA positive control with 10 pg - 1 ng Control Template. B, two-step qHDA positive control using 500 fg - 5 ng, in triplicate.?