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商品編號


M0275S-1,600 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




1,600 units


Concentration

8,000 units/ml

商品信息

Description:

Bst
DNA Polymerase, Large Fragment is the portion of the
Bacillus
stearo
Thermo
philus
DNA Polymerase protein that contains the 5? → 3? polymerase activity, but lacks 5? →3? exonuclease activity.
Highlights
Isolated from a recombinant source
Sequencing through problematic secondarystructures
Supplied with 10X Reaction Buffer
Product Source
Bst
?Polymerase, Large Fragment is prepared from an?
E. coli
?strain containing a genetic fusion of the?
Bacillus stearo
Thermo
philus
?DNA Polymerase gene, lacking the 5? → 3? exonuclease domain, and the gene coding for
?E. coli?
maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion of the fusion is cleaved off?in vitro. The remaining polymerase is purified free of MBP (1).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Thermo
Pol
??
Reaction Buffer Pack
-20
10X
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Notes:
Bst DNA Polymerase does not exhibit 3?→ 5? exonuclease activity.100 ?g/ml BSA or 0.1%Triton X-100 is required for long term storage.Reaction temperatures above 70°C are not recommended.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR.