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商品編號


T1030S-250 preps



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Nucleic Acid Purification




250 preps






商品信息

Description:
?
The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustain
ABI
lity in mind, Monarch kits use significantly less plastic and respons
IBL
y-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides.
View our videos on protocols, tips, and recycling Monarch.
?
APPLICATIONS
PCR cleanup
DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.
Enzymatic reaction cleanup
Modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed allowing efficient desalting and concentration of the DNA sample.
CDN
A cleanup
DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.
Labeling cleanup
Unincorporated r
ADI
olabeled or fluorescently labeled nucleotides can be removed from the DNA substrate
Plasmid cleanup
Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.
Oligonucleotide cleanup
ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥?15 bp) can be purified using the Oligonucleotide Cleanup Protocol.
Monarch DNA Cleanup Column Design
Monarch columns are designed for performance
Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
?Advantages:
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH
Buffers and columns available separately
Significantly less plastic used when compared with other kits?
Respons
IBL
y-sourced and recyclable packaging?
Specifications
DNA Sample Type:
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).*
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb **
Typical Recovery:
DNA (50 bp to 10 kb): 70–90% /?DNA (11–23 kb): 50–70%
Elution Volume:
≥ 6 μl
Purity:
A
260/280
> 1.8 and A
260/230
> 1.8
Protocol Time:
5 minutes of spin and incubation time
Compat
IBL
e Downstream
Applications:
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
* ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol .
** DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
?
Monarch PCR & DNA Cleanup Kit (5 ?g) Protocol?
Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the le
ADI
ng supplier
Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq? Quick-Load? 2X Master Mix (
NEB
#M0486). DNA was eluted in 20 μl (
NEB
) and 40 μl (
Qiagen
) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF? (
NEB
#R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.?
Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples
Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.
Learn More About Monarch
NEB
Monarch.com
Optimize your results with our unique column design
Enhance your DNA purification experience
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Kit Components
The following reagents are supplied with this product:
Store at (°C)
Concentration
Monarch? DNA Cleanup Binding Buffer
25
1X
Monarch? DNA Wash Buffer
25
5X
Monarch? DNA Elution Buffer
25
1X
Monarch? DNA Cleanup Columns (5 μg)
25
Notes:
The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.