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商品編號


S1550S-25 isolations



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


RNA Reagents



Size

25 isolations


商品信息

Description:
The
New England Biolabs
Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)
25
to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)
25
Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand
CDN
A reaction.
Figure 1:
Consistency and wide isolation range are demonstrated by poly(A)+ RNA isolation from duplicate samples of decreasing numbers of HEPG2 cells (5 x 105 to 1 x 103) by direct lysis/binding in microtiter plate followed by mRNA isolation with the magnetic method. 1/10th of isolated mRNA is converted to oligo (dT) primed
CDN
A using ProtoScript First Strand
CDN
A Synthesis Kit (
NEB
#E6500) and qPCR done with validated primers for the peptidylpropyl isomerase, a low-abundance housekeeping gene.
Figure 2:
Facilitate the isolation of poly(A)+ RNA using the Magnetic mRNA Isolation kit
Kit Components
The following reagents are supplied with this product:
Store at (°C)
Concentration
Oligo d(T)
25
Magnetic Beads
4
5 mg/ml
Lysis/Binding Buffer
1X
Low Salt Buffer
1X
Wash Buffer I
1X
Wash Buffer II
1X
Elution Buffer
1X