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The kit is for the quantitative level of SPARC in the sample, adopt purified Human SPARC to coat microtiter plate, make solid-phase antibody, then add SPARC to wells, Combine SPARC antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of SPARC in the samples is then determined by comparing the O.D. of the samples to the standard curve.